Product Description
UltraNuclease, also known as non restrictive endonuclease or broad-spectrum nuclease; It is a non-specific endonuclease derived from Serratia Marcescen that can cleave between any nucleotide in the chain, completely digesting the nucleic acid into 5 '- monophosphate oligonucleotides with 2-5 base lengths. It can degrade various forms of (double stranded, single stranded, linear, circular, natural or denatured) DNA and RNA under very wide conditions (6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF), Widely used to remove nucleic acids from biological products. Subsequently, UCF. ME can also be removed through corresponding methods UltraNuclease.
This kit applies the principle of double antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residue of denatured and non denatured UltraNuclease omnipotent nucleases. Rabbit polyclonal antibodies against UltraNuclease are coated on the microplate to form a solid phase antibody. UltraNuclease standards and test samples are added to the solid phase antibody microplate, and then biotin labeled Anti UltraNuclease polyclonal antibodies are added, Finally, horseradish peroxidase labeled streptavidin (SA-HRP) was added to form an antibody+antigen+antibody Biotin+SA-HRP complex. After washing, TMB substrate was added for color development; TMB is converted into blue under the catalysis of HRP enzyme and ultimately into yellow under the action of acid. The color depth is positively correlated with the amount of UltraNuclease in the sample. The quantitative range of this test kit is 0.047-3 ng/mL; Lower detection limit: 23.5 pg/mL.
Transportation and preservation methods
Ice bag (wet ice) transportation. Products stored at 4 ℃ and not unpacked are valid for one year. After opening the reagent, the validity period is six months. Never freeze.
The storage time of the configured reagent: 1) Diluted washing buffer and dilution buffer, stored at 4 ℃, with an expiration date of 1 week; 2) Standard 36701-C is liquid, stored at 4 ℃, and has a validity period of 1 year; 3) The prepared detection antibody and termination buffer are stored at 4 ℃ and have a validity period of 1 month.
matters needing attention
1) The reagent kit needs to be used within its shelf life.
2) It is prohibited to mix different batches of related reagents.
3) This product can only be used to detect the target antigens and samples marked in the instruction manual. Other applications need to be validated by user design and evaluated for reliability and accuracy based on the results.
4) This product is only for research purposes and cannot be used for clinical diagnosis or treatment.
5) For your safety and health, please wear laboratory clothes and disposable gloves to exercise
